Composite

Part:BBa_M36056

Designed by: Kara Helmke Rogers   Group: Stanford BIOE44 - S11   (2016-10-24)


Thiosulfate Sensor (ThsS)

ThsS is a sensor designed to detect thiosulfate, an intermediate of hydrogen sulfide. It utilizes a natural biological thiosulfate detection system adapted from a bacterium, Schewanella halifaxensis, for use in E. coli by Jeffrey Tabor's lab in 2017. ThsS combines a transmembrane thiosulfate sensor (SHAL3128) and a transcriptional activator (SHAL3129) which becomes phosphorylated by the membrane sensor to induce transcription at a specific promoter site, PphsA342. Because the sensor sequence is almost 2,000 base pairs long, we separated the PphsA342 promoter/reporter system on another plasmid, ThsR (part BBa_M50100).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1208
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 607
    Illegal BamHI site found at 655
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1140


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Categories
Parameters
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